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For low-dose starters (2.5mg), you are effectively subsidizing high-dose users, as similar 2.5mg doses can be found for around $160 per month elsewhere
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Cells were incubated for 30 min at 37 °C and then lysed in lysis buffer containing cAMP-d2 and cAMP cryptate (Cisbio) for 1 h at room temperature
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